ABCG2 Mouse Monoclonal Antibody [Clone ID: BXP-34]

Specifications

Product Data
Clone Name	BXP-34
Applications	IF, IHC, WB
Recommend Dilution	Flow Cytometry: 1/20 (Permeabilisation required). Immunocytochemistry: Use 1/20-1/50 on Acetone Fixed Cytospin preparations. Immunohistochemistry on Frozen Sections: 1/20 on Acetone Fixed Frozen Sections can be followed by incubation with Rabbit anti-Mouse IgG (1/25) and and a Monoclonal mouse APAAP complex (1/50). This antibody BXP-34 is unreactive in Western blot and cannot be used on Formaldehyde-Fixed Paraffin-Embedded Human tissues and tumours.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Immunization with the mitoxanthrone resistant, BCRP overexpressing cell line MCF7 MR.
Specificity	This Monoclonal antibody BXP-34 reacts with an internal epitope of BCRP, a 70 kD transmembrane half-transporter which is involved in multidrug resistance. It did not cross-react with the Human MDR1, MRP1, MRP2, MRP5 gene products.
Isotype	IgG1
Formulation	State: Liquid Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP binding cassette subfamily G member 2 (Junior blood group)
Database Link	Entrez Gene 9429 Human UniProt ID Q9UNQ0
Background	The breast cancer resistance protein (BCRP/ABCG2) is a member of the ATP-binding cassette family of drug transporters and confers resistance to various anticancer drugs. The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
Synonyms	Breast cancer resistance protein 1, ABCP, MXR
Reference Data

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