Abcg2 Rat Monoclonal Antibody [Clone ID: BXP-9]

Specifications

Product Data
Clone Name	BXP-9
Applications	IF, IHC, WB
Recommend Dilution	Western blotting: Use 1/20-1/50 dilution and HRP-anti-Rat-IgG. Flow Cytometry (optimal conditions still to be defined). Immunocytochemistry: Use 1/20-1/50 on Acetone Fixed Cytospin preparations. Immunohistochemistry on Frozen Sections: 1/20 on Acetone Fixed Frozen Sections can be followed by incubation with Biotin-labeled Rabbit anti-Rat IgG (1/100) and HRP-labeled streptavidin (1/ 500).
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Fusion protein containing the E. coli maltose binding protein and a fragment of the Mouse bcrp protein corresponding to amino acids 221-394.
Specificity	This Monoclonal antibody clone BXP-9 reacts with an internal epitope of bcrp, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. It does not react with the Human BCRP molecule.
Isotype	IgG1
Formulation	State: Supernatant State: Serum Free Culture Supernatant Stabilizer: 0.7% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP-binding cassette, sub-family G (WHITE), member 2
Database Link	Entrez Gene 26357 Mouse UniProt ID Q7TMS5
Background	The breast cancer resistance protein (BCRP/ABCG2) is a member of the ATP-binding cassette family of drug transporters and confers resistance to various anticancer drugs. The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
Synonyms	Breast cancer resistance protein 1, ABCP, MXR
Reference Data

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