CD45 / LCA Rat Monoclonal Antibody [Clone ID: IBL-5/25]

Specifications

Product Data
Clone Name	IBL-5/25
Applications	FC
Recommend Dilution	Immunohistochemistry on aceton-fixed frozen sections. Immunoprecipitation. Flow Cytometry. TEST RESULTS: Mouse Strain: BALB/c Cell Concentration: 1x10e6 cells per tests Antibody Concentration Used: 0.1 μg/10e6 cells, Isotypic Control: PE-Rat IgG1 Cell Source Percentage of cells stained above control: Mesentric Lymph Node 99.8%
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	IL-3 dependent mast cells derived from WB- +/+ mice Donor: Wistar spleen Fusion Partner: X63.653. Ag8
Specificity	Anti-mouse CD45 monoclonal antibody detects CD45 (L-CA) which is a transmembrane phosphotyrosine phosphatase expressed on leukocytes. This mAb induces the in vitro clustering of mouse lymphocytes (both T and B cells).
Isotype	IgG1
Formulation	PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: PE State: Liquid purified Ig fraction Label: R - Phycoerythrin
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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