TCR DO-11.10 Mouse Monoclonal Antibody [Clone ID: KJ1-26]

Specifications

Product Data
Clone Name	KJ1-26
Applications	FC
Recommend Dilution	Flow Cytometry (See Protocols). This clone has also been reported to work in Immunoprecipitation, ELISA and Inhibition Assay.
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	T cell hybridoma, D011.10. Donor: Mouse ascites fluid.
Specificity	This Monoclonal Antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested. (1) The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-Ad major histocompatibility molecules. (1,2) The use of DO11.10 and Clone KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity. (3-6) The DO11.10 T cell hybridoma is available from the laboratories of Drs. Kappler and Marrack. (2,7)
Isotype	IgG2a
Formulation	PBS containing 0.02% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Protein G Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	DO-11.10 T cell Receptor
Note	Protocol: Test Results (See Figure 1.) Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/c-[Tg] D011.10 TCR-[KO] RAG2. Cell Concentration : 1x10e6 cells per tests. Antibody Concentration Used: 0.5 µg/10e6 cells. Isotypic Control (Shaded): Purified Mouse IgG2a.
Reference Data

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