Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: 131-14871 (AH4H7-1)]

Specifications

Product Data
Clone Name	131-14871 (AH4H7-1)
Applications	FC, IHC
Recommend Dilution	Flow Cytometry. Immunohistochemistry.
Host	Mouse
Clonality	Monoclonal
Specificity	Bromodeoxyuridine (BrdU), a thymidine analogue, is incorporated into DNA during replication. This antibody reacts with cells that incorporated BrdU during proliferation activity in a variety of specimens such as tumors, blood, cell lines and tissues.
Isotype	IgG1
Formulation	10mM Tris, pH 7.5 containing 150mM Sodium Chloride State: Purified State: Liquid purified IgG fraction (> 90% pure by SDS-PAGE). Product is 0.2 µm filtered. Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
Reference Data

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