CLIC5 (Isoform 1) (160-173) Mouse Monoclonal Antibody [Clone ID: CLIC5-02]

Specifications

Product Data
Clone Name	CLIC5-02
Applications	FC, WB
Recommend Dilution	Flow cytometry. Western blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a
Specificity	This antibody recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Cross reactivity with CLIC5b was not determined.
Isotype	IgG2b
Formulation	Phosphate buffered saline (PBS) State: Purified State: Liquid Ig fraction Preservative: 15 mM sodium azide, approx. pH 7.4
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	chloride intracellular channel 5
Database Link	Entrez Gene 53405 Human UniProt ID Q9NZA1
Background	CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.
Synonyms	Chloride intracellular channel protein 5
Note	Protocol: 1. Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503.
Reference Data

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