Nck (NCK1) (2nd SH3 dom.) Mouse Monoclonal Antibody [Clone ID: EM-06]

Specifications

Product Data
Clone Name	EM-06
Applications	IF, WB
Recommend Dilution	Western blot: 2 μg/ml. Positive control: Jurkat cell lysate. Immunocytochemistry: 2 μg/ml. Positive control: Human NCK1-transfected COS-7 cells.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant second SH3 domain of human Nck1 fused to GST
Specificity	This antiibody recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.
Formulation	Phosphate buffered saline (PBS), approx. pH 7.4 State: Purified State: Liquid Ig fraction Preservative: 15 mM sodium azide
Concentration	lot specific
Purification	Protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	NCK adaptor protein 1
Database Link	Entrez Gene 4690 Human UniProt ID P16333
Background	NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.
Synonyms	Cytoplasmic protein NCK1, NCK, NCK adaptor protein 1
Reference Data

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