6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: 10A12]

Specifications

Product Data
Clone Name	10A12
Applications	WB
Recommend Dilution	Western blot.
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic 6 x Histidine peptide conjugated with KLH
Specificity	This antibody binds to His-tagged recombinant protein, but not with KLH.
Isotype	IgG1
Formulation	0.01M PBS pH 7.2 State: Purified State: Lyophilized purified IgG fraction
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.
Reference Data

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