Siglec-H Rat Monoclonal Antibody [Clone ID: 440c]

Specifications

Product Data
Clone Name	440c
Applications	FN, IHC
Recommend Dilution	Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50. Flow cytometry: The typical starting working dilution is 1:50. Functional assays (inhibition).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). It exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.
Isotype	IgG2b
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	sialic acid binding Ig-like lectin H
Database Link	Entrez Gene 233274 Mouse UniProt ID Q3Y597
Background	Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo.
Synonyms	6430529G09Rik; Siglec-H
Reference Data

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