Emcn Rat Monoclonal Antibody [Clone ID: V.7C7.1]

Specifications

Product Data
Clone Name	V.7C7.1
Applications	IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Immunoprecipitation. Flow Cytometry: The typical starting working dilution is 1/50. Western blot: The typical starting working dilution is 1/50. Not suitable for Functional assays.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse endomucin-IgG fusion protein
Specificity	The monoclonal antibody V.7C7.1 recognizes endomucin, type I membrane protein of 248 amino acids (75 kDa) and shows no signicant homology to any known glycoprotein.
Isotype	IgG2a
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	endomucin
Database Link	Entrez Gene 59308 Mouse UniProt ID Q9R0H2
Background	As a typical mucin-like glycoprotein, endomucin has a high content of serine and threonine residues, suggesting strong O-glycosylation; the sensitivity to O-sialoglycoprotein endopeptidase indicates that endomucin is also a sialomucin. Endomucin is an endothelial-specific sialomucin. It is a constitutively expressed endothelial cell surface protein that is found on all venules but is absent from high endothelial venule cells (HEV) of peripheral and mesenteric lymph nodes as well as Peyer's patches, the specialized site for most efficient lymphocyte trafficking. This could indicate an anti-adhesive function of endomucin, as demonstrated for other sialomucins. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) is another cell adhesion molecule that contains a mucin-like domain and is expressed on HEV in Peyer's patches, mesenteric lymph nodes and on venules in intestinal lamina propria. In the HEV of mesenteric lymph nodes, the mucin-like domain of a subpopulation of MAdCAM-1 molecules contains sulfated carbohydrate side chains that interact with L-selectin. The presence of three putative protein kinase C phosphorylation sites in the cytoplasmic tail of endomucin indicates that endomucin has the capacity to be a signaling molecule.
Synonyms	EMCN, EMCN2, MUC14, Endomucin-2, Mucin-14, Gastric cancer antigen Ga34
Reference Data

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