TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 4H31]

Specifications

Product Data
Clone Name	4H31
Applications	ELISA, FN, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on frozen sections: The typical starting working dilution is 1:10. Flow cytometry: The typical starting working dilution is 1:10. Functional assays. Immunoassays. Immunoprecipitation. Western blot: The typical starting working dilution is 1:10. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Specificity	The antibody reacts with human native and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant TNF-alpha as determined with L929 cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF-alpha and lacks crossreactivity with human lymphotoxin.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 7124 Human UniProt ID P01375
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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