Complement C4A (C4A) (alpha 2 chain) Mouse Monoclonal Antibody [Clone ID: 7H4]

Specifications

Product Data
Clone Name	7H4
Applications	ELISA, IHC, WB
Recommend Dilution	Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50. Immunoassay. Western blot: The typical starting working dilution is 1:50.
Reactivity	Guinea Pig, Human
Host	Mouse
Clonality	Monoclonal
Specificity	The monoclonal antibody 7H4 (also known as clone M4d2) recognizes an epitope on the alpha 2 chain of human C4d. It recognizes C4, C4b and C4d.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	complement component 4A (Rodgers blood group)
Database Link	Entrez Gene 720 Human UniProt ID P0C0L4
Background	Complement factor C4 (MW 41 kDa), formerly known as Gg protein, consists of an alpha-, beta- and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved by factor I resulting in C4c and C4d, in which step all functional sites are lost. The C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. The thioester formed between the side chains of Cys1010 and Gln1013 within the C4d region of the α-chain mediates covalent attachment to the target surface bearing activated forms of C1s or MASP. Furthermore, C4d is highly homologous to C3d with over 35% shared amino acid sequence.In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.
Synonyms	Complement component 4, CPAMD2, CPAMD3, Acidic complement C4, Basic complement C4
Reference Data

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