Caveolin-1 Mouse Monoclonal Antibody [Clone ID: 7C8]

Specifications

Product Data
Clone Name	7C8
Applications	IF, IP, WB
Recommend Dilution	Immunoflourescence (1,3): Reduced sample treatment and SDS-PAGE was used. The band sizes are ~22 kDa (Caveolin-1β) and ~25 kDa (caveolin-1α) (Ref.1). Immunoprecipitation (1). Western blot (1,2).  The typical starting working dilution is 1:50. Positive control: Adipocytes (3T3-L1 adipocytes).
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes (Sprague Dawley) (Ref 4)
Specificity	This antibody recognizes rat caveolin-1, a membrane protein of ~22 kDa. It recognizes caveolin-1α as well as caveolin-1β, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat. The antibody can be used to immuno-isolate caveolae.
Isotype	IgG2b
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Database Link	UniProt ID P41350
Background	Caveolae are sphingomyelin/cholesterol-rich membrane domains first discovered as membrane invaginations on the surface of endothelial and epithelial cells. Caveolae are present in most cells, but are especially abundant in adipocytes. In addition to caveolins only two major protein components of caveolae were identified, namely the semicarbazide sensitive amine oxidase (SSAO) and the scavenger receptor CD36. Caveolin cycles between the plasma membrane and intracellular compartments via the endocytotic pathway. Caveolin is involved in the rapid intracellular transport of newly synthesized cholesterol from the ER directly to the caveolae. Caveolin plays an important role in multiple signaling pathways, molecular transport and cellular proliferation and differentiation. Caveolin binds to endothelial nitric oxide synthase leading to enzyme inhibition. Furthermore caveolin is a candidate tumor suppressor gene in many tumors. The specific functions of caveolin-1/caveolae are highly cell and context dependent.
Synonyms	CAV1
Reference Data

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