Sftpd Mouse Monoclonal Antibody [Clone ID: VIF9]

Specifications

Product Data
Clone Name	VIF9
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (detection). Immunohostochemistry on paraffin sections of rat lung: Typical starting working dilution is 1:10. Cannot be used for staining paraffin sections of human lung! Western blot: Typical starting working dilution is 1:10.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Specificity	Monoclonal antibody VIF9 specific for rat surfactant protein D shows significant cross reactivity with human SP-D.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	surfactant protein D
Database Link	Entrez Gene 25350 Rat UniProt ID P35248
Background	SP-D belongs to the collectin familiy. These proteins are oligomeric proteins composed of carbohydrate-recognition domains (CRD) attached to collagenous regions. They are structurally similar to the ficolins although they make use of different CRD structures: C-type lectin domain for the collectins. The anti-microbial effector mechanisms of SP-D are direct opsonization, neutralization, and agglutination. Thus limiting the infection and concurrently orchestrating the subsequent adaptive immune response. The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type II cells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointeststinal tract and in epithelial cells of exocrine glands. SP-D synthesis and secretion increase significantly after inflammatory stress. Increased amounts of SP-D in lavage and tissue, particularly in type II pneumocytes, in Clara cells and in hyperplastic goblet cells are found in inflamed lungs. The localization of SP-D in endocytic vesicles and in lysosomal granules of alveolar macrophages suggests that a receptor-mediated uptake occurs. SP-D binds to apoptotic neutrophils and enhances their clearance by alveolar macrophages.
Synonyms	Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7
Reference Data

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