CD62E (SELE) Mouse Monoclonal Antibody [Clone ID: ENA2]

Specifications

Product Data
Clone Name	ENA2
Applications	ELISA, FN, IHC
Recommend Dilution	Immunohistochemistry on frozen sections. Functional assays. Immunoassays. For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 μg/ml) in tissue culture medium at 37˚C. As negative control it is advised to use a control murine IgG1 antibody.For use in adhesion studies dilutions have to be made according to the amounts of E-Selectin to be inhibited. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). It reacts with human endothelial cells activated with TNF-alpha, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the E-selectin gene. It inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	selectin E
Database Link	Entrez Gene 6401 Human UniProt ID P16581
Synonyms	SELE, ELAM1, LECAM2
Reference Data

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