CD3E Mouse Monoclonal Antibody [Clone ID: CRIS-7]

Specifications

Product Data
Clone Name	CRIS-7
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on frozen sections: 0.5mg/ml (1:400). Suggested positive control: Human tonsil. Has been described to work in FACS. Does not react in Immunohistochemistry on paraffin fixes cells (epitope is not fixation resistant).
Reactivity	Human, Monkey, Rhesus Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Stimulated human leukocytes
Specificity	This antibody detects CD3 antigen on mature human T-cells.
Isotype	IgG2a
Formulation	Phosphate buffered saline pH 7.2 (PBS) Label: Biotin State: Lyophilized Ig fraction Stabilizer: 5 mg/ml bovine serum albumin (BSA) Preservative: 0.05% (v/v) Kathon CG
Reconstitution Method	Reconstitute by adding 0.5ml distilled water.
Concentration	0.2mg/ml (reconstituted)
Purification	Affinity purified
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD3e molecule
Database Link	Entrez Gene 916 Human UniProt ID P07766
Background	The CD3 antigen is a protein complex composed of four distinct chains (CD3γ, CD3δ and two CD3ε) that associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. The TCR, ζ-chain and CD3 molecules together comprise the TCR complex. The CD3γ, CD3δ and CD3ε chains are highly related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain.
Synonyms	T3/Leu-4
Reference Data

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