CD34 Mouse Monoclonal Antibody [Clone ID: QBEnd-10]

Specifications

Product Data
Clone Name	QBEnd-10
Applications	FC, FN, IHC, IP, WB
Recommend Dilution	Flow cytometry: 5-10 μg/ml. Immunoprecipitation. Western blot. Immunohistochemistry on paraffin sections: 1-2 μg/ml. Immunohistochemistry on frozen sections. Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Human endothelial vesicles
Specificity	This antibody reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
Formulation	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized State: Azide Free State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD34 molecule
Database Link	Entrez Gene 947 Human UniProt ID P28906
Background	CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Synonyms	Hematopoietic progenitor cell marker
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Hematopoietic cell lineage

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