Cd4 Rat Monoclonal Antibody [Clone ID: GK1.5]

Specifications

Product Data
Clone Name	GK1.5
Applications	FC, FN, IF, IHC, IP
Recommend Dilution	Flow cytometry: 1 μg/million cells. Immunoprecipitation: 1-2 μg/100-500 μg of protein in 1 ml lysate. Immunohistochemistry (frozen sections). Immunocytochemistry: 1-4 μg/ml. Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse CTL clone V4 cells
Specificity	This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Isotype	IgG2b
Formulation	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized State: Low Endotoxin State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD4 antigen
Database Link	Entrez Gene 12504 Mouse UniProt ID P06332
Background	CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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