alpha Actinin (ACTN1) Mouse Monoclonal Antibody [Clone ID: SA-20]

Specifications

Product Data
Clone Name	SA-20
Applications	IF, IHC, IP, WB
Recommend Dilution	Western Blot: 0.25-0.5 μg/ml. Immunohistochemistry on paraffin* sections: 0.5 - 1.0 μg/ml (*Boiling the sections is required). Immunohistochemistry on Frozen 1.0 - 2.0 μg/ml
Reactivity	Human, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rabbit skeletal alpha-actinin
Specificity	This antibody reacts to Alpha-actinin-1.
Formulation	1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative State: Purified State: Lyphilized purified Ig fraction
Reconstitution Method	Restore with 1.2% sodium acetate or neutral PBS
Concentration	0,1 mg/ml (after reconstitution with PBS)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	actinin alpha 1
Database Link	Entrez Gene 81634 RatEntrez Gene 109711 MouseEntrez Gene 87 Human UniProt ID P12814
Background	Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
Synonyms	Alpha actinin cytoskeletal isoform, Non-muscle alpha-actinin 1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways	Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction

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