Lactoferrin (LTF) Mouse Monoclonal Antibody [Clone ID: NI 25]

Specifications

Product Data
Clone Name	NI 25
Applications	ELISA, IF, IP, WB
Recommend Dilution	To identify the presence of Lactoferrin in Human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect Immunofluorescence staining and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/20 upwards. ELISA: from 1/100 upwards. Western blot: from 1/250 upwards.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified lactoferrin isolated from Human milk.
Specificity	The reactivity of the antiserum is restricted to Lactoferrin as tested in indirect binding Immunoblotting and Indirect Immunoperoxidase staining. The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation	PBS, pH 7.2 without preservatives or foreign proteins Label: HRP State: Lyophilized purified Ig fraction Label: Horseradish Peroxidase Molar radio: Peroxidase/IgG ~1.7
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water
Concentration	lot specific
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Gene Name	lactotransferrin
Database Link	Entrez Gene 4057 Human UniProt ID P02788
Background	Human lactoferrin is a 78kD globular protein found in body secretions such as milk and saliva and is a member of the transferrin family proteins. Transferrins control the level of free iron in the body, by binding and transporting iron. Lactoferrin does not appear to have a transporting function, instead it has antimicrobial activity, by keeping the levels of iron in body fluids low and thus preventing microbes from acquiring iron. Removing free iron from body fluids and inflamed areas also serve to protect against the damaging effects of free radicals. Lactoferrin is used as a therapeutic and prophylactic treatment of various illnesses, such as cancers and intestinal illnesses. May be used to detect lactoferrin in ELISA assays.
Synonyms	LTF, LF, Lactoferrin, EC=3.4.21, Talalactoferrin
Reference Data
Protein Families	Druggable Genome, Protease, Secreted Protein

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