Human Kappa Light Chain (free and bound) Mouse Monoclonal Antibody [Clone ID: NI 285, NI 250]

Specifications

Product Data
Clone Name	NI 285, NI 250
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, Indirect Immunoperoxidase staining and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified Bence Jones kappa proteins isolated from pooled Human urine.
Specificity	The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species. The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding Enzyme Immunoassay, Imunoblotting, Immunoprecipitation and Direct Immunoperoxidase staining.
Isotype	IgG1
Formulation	State: Azide Free State: Lyophilized (Delipidated, heat inactivated) stable whole ascites without preservatives and foreign proteins.
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.
Reference Data

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