Heparan-Sulfate-Proteoglycan Mouse Monoclonal Antibody [Clone ID: 2E2/B5]

Specifications

Product Data
Clone Name	2E2/B5
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (less than 1 µg/ml). Western blot (1 µg/ml). Immunohistochemistry (1 µg/ml)
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG)
Specificity	This Monoclonal antibody is specific for a core protein epitope of a Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG). AM20231PU recognizes an epitope different from that recognized by clones 1F10/B8 (Cat.No AM02102PU) and 2H7/G11 (Cat.-No AM02103PU).
Isotype	IgG1
Formulation	50 mM TRIS pH 7.4 State: Purified State: Lyophilized purifed IgG fraction from Cell Culture Supernatant
Reconstitution Method	Restore in aqua bidest to 1 mg/ml
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistoche-mistry to the basement membrane. Amino acid sequence ana-lysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
Synonyms	small Heparan sulfate proteoglycan core protein
Reference Data

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