Streptavidin Mouse Monoclonal Antibody [Clone ID: 36G3]

Specifications

Product Data
Clone Name	36G3
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 0.05-1 µg/ml.
Host	Mouse
Clonality	Monoclonal
Immunogen	Streptavidin
Specificity	This monoclonal antibody (Clone 36G3) binds the Tetrameric Steptavidin in liquid phase and in Immunoblot applications. The antibody has a very low affinity for monomeric Streptavidin.
Isotype	IgG1
Formulation	1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Database Link	UniProt ID P22629
Background	Streptavidin is a tetrameric protein that binds tightly to a small growth factor Biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the Vitamin Biotin; the dissociation constant (Kd) of the Biotin-Streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of Biotin and biotinylated molecules has made Streptavidin one of the most important components in diagnostics and laboratory kits.
Synonyms	StAv
Note	Molecular Weight: 60 kDa (Tetramer)
Reference Data

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