NANOG Mouse Monoclonal Antibody [Clone ID: 60CT70.5.12]

Specifications

Product Data
Clone Name	60CT70.5.12
Applications	IHC, WB
Recommend Dilution	ELISA: 1/1,000. Western blot: 1/1,000. Immunohistochemistry: 1/10-1/50.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody is generated from mouse immunized with Human recombinant NANOG protein.
Specificity	This antibody is specific to Human NANOG.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	34620 Da
Gene Name	Nanog homeobox
Database Link	Entrez Gene 79923 Human UniProt ID Q9H9S0
Background	NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Synonyms	FLJ12581; FLJ40451; hNanog
Reference Data

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