GST-Tag Mouse Monoclonal Antibody [Clone ID: 9AT106]

Specifications

Product Data
Clone Name	9AT106
Applications	WB
Recommend Dilution	ELISA: 1/3,000. Western blot: 1/2,000.
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant GST fusion protein was used to produced this monoclonal antibody.
Isotype	IgG1
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08515
Background	Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner.Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Synonyms	Glutathione S-transferase Tag, GST26-Tag
Reference Data

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