6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: 6AT18]

Specifications

Product Data
Clone Name	6AT18
Applications	WB
Recommend Dilution	Peptide ELISA: 1/5,000. Western blotting: 1/1,000.
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
Isotype	IgG1
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative. State: Ascites State: Ascites.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Reference Data

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