HA Epitope Tag (YPYDVPDYA) Mouse Monoclonal Antibody [Clone ID: 12CA5]

Specifications

Product Data
Clone Name	12CA5
Applications	WB
Recommend Dilution	ELISA: 1/1,000. Western blotting: 1/1,000. ChIP (See Ref.1). Immunoprecipitation (See Ref.2).
Host	Mouse
Clonality	Monoclonal
Immunogen	KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
Specificity	This monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
Isotype	IgG2b
Formulation	State: Ascites State: Liquid Preservative: 0.09% (W/V) Sodium Azide
Concentration	lot specific
Purification	Protein G column, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Epitope tags consisting of short sequences recognized by well-characterizated Monoclonal Antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples.
Reference Data

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