HCV NS4 Mouse Monoclonal Antibody [Clone ID: 7B7/5G9]

Specifications

Product Data
Clone Name	7B7/5G9
Applications	ELISA, WB
Recommend Dilution	ELISA: React with Human Hepatitis C Virus. Western Blot: Use of HCV NS4 antibody (clone 7B7/5G9) at 0.5 µg/ml will allow visualization of 0.5-10 µg/lane of synthetic NS-4 peptide and 0.1 µg/lane of Recombinant Chimeric HCV polyprotein. Testing is under both reducing and non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic polypeptide in which overlapping sequence of at least 90 residues in length were selected from immunodominant NS-4 region of Chinese HCV strains.
Specificity	The HCV NS4 antibody (clone 7B7/5G9) reacts with Synthetic NS-4a Protein (aa 1689-1735) and Recombinant Chimeric HCV Polyprotein (60 kDa). No cross reaction can be seen with HCV capsid region and other non-structural regions.
Isotype	IgG1
Formulation	0.01M PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
Synonyms	Hepatitis C Virus, NS4B, Non-structural protein NS4A
Reference Data

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