HCV NS3 Mouse Monoclonal Antibody [Clone ID: 281]

Specifications

Product Data
Clone Name	281
Applications	ELISA, WB
Recommend Dilution	ELISA. Western Blot: Use of Hepatitis C Virus NS3 antibody (clone 281) at 0.1-1 µg/ml will allow visualization of 100 ng/lane of both Recombinant Chimeric HCV Polyprotein and Recombinant NS-3 Protein. No reaction can be seen with Synthetic NS-3. Testing is under reducing and non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Recombinant Chimeric HCV Polyprotein (555 a.a).
Specificity	HCV NS-3 antibody (clone 281) reacts with Recombinant NS-3 (aa 1252-1477), Synthetic NS-3 (aa 1378-1458), and Recombinant Chimeric HCV Polyprotein (60 kDa) Cross-Reactivity: No cross reaction can be seen with Recombinant Capsid Protein C (CPC)+Envelope Protein M (EPM) (core) (aa 1-142), Synthetic CPC (aa 1-61), and Synthetic NS-4a Protein (aa 1689-1735).
Isotype	IgG1
Formulation	0.01M PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
Synonyms	Hepatitis C Virus Serine proteiase NS3
Reference Data

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