HCV Core protein Mouse Monoclonal Antibody [Clone ID: B2]

Specifications

Product Data
Clone Name	B2
Applications	ELISA, WB
Recommend Dilution	ELISA: React with Human Hepatitis C Virus core protein, and no reaction with HCV non-structure protein. Western Blot: Use of anti-HCV Core Protein antibody at 0.1-0.5 µg/ml will allow visualization of 0.1 µg/lane of recombinant Capsid Protein (CPC)+Envelope protein M (EPM) (core), 0.5 µg/lane synthetic CPC, and 0.1 µg/lane recombinant chimeric HCV polyprotein. Testing is under both reducing and non-reducing conditions. Has been used successfully in the recognition of in-vitro translated HCV core protein.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptides derived from HCV Capsid Protein.
Specificity	This HCV Core Protein antibody is reactive with recombinant Capsid Protein CPC+Envelope protein M (EPM) (core) (1st-142th amino acid), synthetic CPC (1st-61st amino acid), and recombinant chimeric HCV polyprotein (60kDa).
Isotype	IgG1
Formulation	0.01M PBS, pH 7.0 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Purification	Affinity chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
Synonyms	Hepatitis C Virus core protein
Reference Data

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