FKBP52 (FKBP4) Mouse Monoclonal Antibody [Clone ID: AT4D3]

Specifications

Product Data
Clone Name	AT4D3
Applications	ELISA, FACS, ICC/IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Cell lysates of HeLa (40 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FkBP4 (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Immunohistochemistry on Paraffin Embedded Tissue (1/50-1/100). Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-Human FKBP4 (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human FkBP4 (1-459aa) purified from E. coli
Specificity	The antibody recognizes Human FKBP4. Other species not tested.
Isotype	IgG2b, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	FK506 binding protein 4
Database Link	NP_002005 Entrez Gene 2288 Human UniProt ID Q02790
Background	FkBP4, also known as FkBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes.
Synonyms	FK506 binding protein 4, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, HBI, Hsp 56, HSP binding immunophilin, p52, p59 protein, Peptidylprolyl cis trans isomerase, PPIase, Rotamase, T cell FK506 binding protein 59kD
Reference Data
Protein Families	Druggable Genome

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