NFAT2 (NFATC1) Mouse Monoclonal Antibody [Clone ID: AT1C3]

Specifications

Product Data
Clone Name	AT1C3
Applications	ELISA, FACS, FC, ICC/IF, IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Flow Cytometry. Immunofluorescence. Immunohistochemistry on Paraffin Sections (10 µg/ml). This NFATC1 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human NFATc1 (428-716aa) purified from E. coli
Specificity	The antibody recognizes Human NFATc1. Other species not tested.
Isotype	IgG2a, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	nuclear factor of activated T-cells 1
Database Link	NP_765978 Entrez Gene 4772 Human UniProt ID O95644
Background	Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.
Synonyms	nuclear factor of activated T-cells, cytosolic component 1 isoform A, NF-ATC, NFATc, NFAT2
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.