14-3-3 theta (YWHAQ) (1-245) Mouse Monoclonal Antibody [Clone ID: AT1A1]

Specifications

Product Data
Clone Name	AT1A1
Applications	ELISA, FC, ICC/IF, IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 µg/ml). This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. Flow cytometry. Immunofluorescence.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human 14-3-3 tau (1-245aa) purified from E. coli
Specificity	The antibody recognizes Human 14-3-3 protein theta (tau) at aa 1-245. Other species not tested.
Isotype	IgG1, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
Database Link	NP_006817 Entrez Gene 10971 Human UniProt ID P27348
Background	The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.
Synonyms	YWHAQ, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta, Protein theta
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection

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