Cofilin 1 (CFL1) Mouse Monoclonal Antibody [Clone ID: AT1C1]

Specifications

Product Data
Clone Name	AT1C1
Applications	ELISA, ICC/IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 µg/ml). This Cofilin 1 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human Cofilin 1 (1-166aa) purified from E. coli
Specificity	The antibody recognizes Human Cofilin-1. Other species not tested.
Isotype	IgG1, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	cofilin 1
Database Link	NP_005498 Entrez Gene 1072 Human UniProt ID P23528
Background	Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Synonyms	18 kDa phosphoprotein(p18), CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.