NANOG (1-154) Mouse Monoclonal Antibody [Clone ID: 5A10]

Specifications

Product Data
Clone Name	5A10
Applications	ELISA, FACS, FC, IHC, WB
Recommend Dilution	ELISA. Western blot (1/500-1/2,000). Flow Cytometry. Immunohistochemistry on Paraffin Sections (1/50-1/100).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human Nanog (1-154aa) purified from E. coli
Specificity	The antibody recognizes NANOG.
Isotype	IgG2a, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	Nanog homeobox
Database Link	NP_079141 Entrez Gene 79923 Human UniProt ID Q9H9S0
Background	Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
Synonyms	Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog
Reference Data

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