LOC396098 Mouse Monoclonal Antibody [Clone ID: 21-1A4]

Specifications

Product Data
Clone Name	21-1A4
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1) Immunoprecipitation. (Ref.1) Immunohistochemistry on Frozen Sections. (Ref.3)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens.
Specificity	This antibody recognizes Chicken Bu-1a alloantigen (Mr. 70 kDa). It does not react with cells from CHA and H.B15 strains by Immunofluorescence.
Isotype	IgG1
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	B6.1
Database Link	Entrez Gene 396098 Chicken UniProt ID Q90746
Background	Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In Chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.
Synonyms	B-Cell Marker
Reference Data

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