CD28 Mouse Monoclonal Antibody [Clone ID: AV7]

Specifications

Product Data
Clone Name	AV7
Applications	FC
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28.
Isotype	IgG1
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: Biotin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD28 molecule
Database Link	Entrez Gene 396249 Chicken UniProt ID P31043
Background	Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative. (Ref.1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (Ref.2)
Synonyms	TP44
Reference Data

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