Cd86 Rat Monoclonal Antibody [Clone ID: GL1]

Specifications

Product Data
Clone Name	GL1
Applications	FC
Recommend Dilution	Flow Cytometry. (Ref.1-7) Immunoprecipitation. (Ref.1) Immunohistochemistry Frozen Sections. (Ref.6) Functional Assays: In vivo and In vitro blocking assays. (Ref.1,3,5)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	LPS-activated mouse B cells.(Ref.1)
Specificity	This antibody recognises CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4. The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells. (Ref.1,3,5)
Isotype	IgG2a
Formulation	PBS without preservatives. State: Low Endotoxin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD86 antigen
Database Link	Entrez Gene 12524 Mouse UniProt ID P42082
Background	CD86 (also known as B7-2) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. (Ref.1-5) CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. (Ref.1,5) CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. (Ref.1-9).
Synonyms	CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1
Reference Data

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