Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]

Specifications

Product Data
Clone Name	1G10
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry. (Ref.2,7) Immunoprecipitation. (Ref.7) Functional Assays: In vivo and In vitro blocking of ligand binding. (Ref.7)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises CD80/B7-1 (Mr. 45-47 kDa). 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).
Isotype	IgG2a
Formulation	PBS without preservatives. State: Low Endotoxin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD80 antigen
Database Link	Entrez Gene 12519 Mouse UniProt ID Q00609
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Synonyms	CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
Reference Data

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