Cd3e Rat Monoclonal Antibody [Clone ID: C363.29B (YCD3-1)]

Specifications

Product Data
Clone Name	C363.29B (YCD3-1)
Applications	FC, FN
Recommend Dilution	Flow Cytometry: Identification and enumeration of CD3+. (Ref.1,2). Functional Assay: In vitro Depletion of CD3+ cells. (Ref.1) In vitro Activation of T cells (accessory cells must be provided). (Ref.1,3,4)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This Low Endotoxin/Azide free antibody is specific to CD3e (Mr. 25 kD). The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)
Isotype	IgG2b
Formulation	PBS without preservatives. State: Low Endotoxin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD3 antigen, epsilon polypeptide
Database Link	Entrez Gene 12501 Mouse UniProt ID P22646
Background	CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
Synonyms	T3/Leu-4
Reference Data

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