Cd5 Rat Monoclonal Antibody [Clone ID: 4H8E6]

Specifications

Product Data
Clone Name	4H8E6
Applications	FC, FN, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Identification and enumeration of CD5+ cells (< / = 1 µg/10e6 cells). (Ref.15) Immunoprecipitation. (Ref.15) Immunohistochemistry on Frozen Sections. (Ref.15) T Cell Activation. (Ref.15) Western blotting. (Ref.15)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody is specific to CD5/Lyt-1 (Mr. 67 kDa)
Isotype	IgG2a
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: Biotin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD5 antigen
Database Link	Entrez Gene 12507 Mouse UniProt ID P13379
Background	CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (Ref.1-3) It has been identified as the major ligand of the B-cell antigen CD72. (Ref.4,5) The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in Ref.6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (Ref.7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (Ref.13,14)
Synonyms	CD5, LEU1
Reference Data

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