NANOG (20-166) Mouse Monoclonal Antibody [Clone ID: 1E6C4]

Specifications

Product Data
Clone Name	1E6C4
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500-1/2000. Immunofluorescence: 1/200-1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
Specificity	Recognizes NANOG/Nanog homeobox
Formulation	State: Ascites State: Ascitic fluid Preservative: 0.03% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	35 kDa
Gene Name	Nanog homeobox
Database Link	Entrez Gene 79923 Human UniProt ID Q9H9S0
Background	Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
Synonyms	FLJ12581; FLJ40451; hNanog
Reference Data

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