Hexokinase 1 (HK1) Mouse Monoclonal Antibody [Clone ID: 4D7]

Specifications

Product Data
Clone Name	4D7
Applications	ELISA, ICC/IF, IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1:1,000). Immunofluorescence (1:1,000). Immunohistochemistry on Paraffin Sections (5 µg/ml): This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human Hexokinase1 (1-917aa) purified from E. coli
Specificity	The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.
Isotype	IgG2a, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	hexokinase 1
Database Link	NP_000179 Entrez Gene 3098 Human UniProt ID P19367
Background	Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.
Synonyms	BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase-A, HK I, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HXK1, mHk1-s, tumor isozyme, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase 4, HK1,HK2, HK3, HK4
Reference Data

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