SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: 15-414]

Specifications

Product Data
Clone Name	15-414
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/100 diluted antibody to label 1x10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/100. Recommended Positive Control: Human tonsil tissue.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Monocyte-derived dendritic cells. Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Specificity	This antibody is specific for CD172a, also known as signal-regulatory protein alpha.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	signal regulatory protein alpha
Database Link	Entrez Gene 140885 Human UniProt ID P78324
Background	CD172a is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
Synonyms	SHP substrate 1, SHPS-1, Sirp-alpha-2, Sirp-alpha-3, MyD-1 antigen, p84, BIT, MFR, MYD1, PTPNS1, SHPS1
Reference Data

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