GAL4 (Activation Domain) Mouse Monoclonal Antibody [Clone ID: 5C8]

Specifications

Product Data
Clone Name	5C8
Applications	IP, WB
Recommend Dilution	Western blot (5-10 µg/ml). Immunoprecipitation.
Reactivity	Yeast
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and Mouse myeloma cells.
Specificity	Recognizes GAL4 derivatives containing the C-terminal activation domain.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) State: Purified State: Liquid purified Ig fraction Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C only. Avoid repeated freezing and thawing.
Database Link	UniProt ID P04386
Background	GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
Synonyms	GAL81
Reference Data

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