MMP3 Mouse Monoclonal Antibody [Clone ID: 1B4]

Specifications

Product Data
Clone Name	1B4
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1-2 µg/ml. Immunohistochemistry on Paraffin Sections: 1-5 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the Human MMP3 protein and mouse myeloma Ag8563 cells.
Specificity	This antibody recognizes Human Matrix Metalloproteinase 3. Other species not tested.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A/G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	50 kDa (Proenzyme)
Gene Name	matrix metallopeptidase 3
Database Link	Entrez Gene 4314 Human UniProt ID P08254
Background	The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including Collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.
Synonyms	Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP3, STMY1
Reference Data

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