6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: IPA2C5.1]

Specifications

Product Data
Clone Name	IPA2C5.1
Applications	WB
Recommend Dilution	Western Blot.
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells.
Specificity	This antibody is effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ. Specific for 6x-His tags only and for His tags no longer than 6 histidines.
Isotype	IgG2a
Formulation	PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Reference Data

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