gamma Tubulin (TUBG1) (434-449) Mouse Monoclonal Antibody [Clone ID: TU-32]

Specifications

Product Data
Clone Name	TU-32
Applications	IF, WB
Recommend Dilution	Western blot (1-2 µg/ml, reducing conditions). Immunocytochemistry (Methanol/acetone fixation required).
Reactivity	Mammalian, Plant, Protozoa
Host	Mouse
Clonality	Monoclonal
Immunogen	Human gamma-Tubulin peptide, amino acids 434-449
Specificity	The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Formulation	PBS, pH~7.4 containing 15 mM Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tubulin gamma 1
Database Link	UniProt ID P23258
Background	The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The γ-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing γ-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Synonyms	Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1
Reference Data

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