Acetylated Lysine Mouse Monoclonal Antibody [Clone ID: 7F8]

Specifications

Product Data
Clone Name	7F8
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Immunohistochemistry. Western blot: 1 µg/ml was sufficient for detection of 20 µg Acetylated Chicken Erythrocyte Histones.
Reactivity	Bovine, Birds
Host	Mouse
Clonality	Monoclonal
Immunogen	Acetylated KLH
Specificity	Detects proteins containing Acetylated lysine residues in ELISA and Western Blots. Does not detect non-Acetylated lysine residues.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide in 50% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).
Synonyms	AcK, acetyl Lysine, acetyl-Lysine
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.