HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: 01G]

Specifications

Product Data
Clone Name	01G
Applications	ELISA, FC, IF, IHC, IP
Recommend Dilution	Flow Cytometry: 5-6 µg/ml. Immunoprecipitation. Immunohistochemistry (frozen sections). Immunocytochemistry. ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Specificity	The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Isotype	IgG1
Formulation	PBS, pH 7.4 with 15 mM sodium azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	major histocompatibility complex, class I, G
Database Link	Entrez Gene 3135 Human UniProt ID P17693
Background	HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
Synonyms	HLA-6.0, HLAG, MHC class I antigen G
Reference Data

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